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Secondary Appointment in CB

Luis Andres Garza, M.D., Ph.D.

Luis Andres Garza, M.D., Ph.D.

David Hackam, M.D., Ph.D.

David Hackam, M.D., Ph.D.

Lewis H. Romer, M.D.

Lewis H. Romer, M.D.

Michael Matunis, Ph.D.

Michael Matunis, Ph.D.

Shukti Chakravarti, Ph.D.

Shukti Chakravarti, Ph.D.

Research Title: Extracellular matrix and tissue homeostasis

Antony Rosen, M.D.

Antony Rosen, M.D.

Pablo Iglesias, Ph.D.

Pablo Iglesias, Ph.D.

Selected Publications:
Artemenko Y, Axiotakis L Jr., Borleis J, Iglesias PA, Deveotes PN. 2016. Chemical and mechanical stimuli act on common signal transduction and cytoskeletal networks. Proc Natl Acad Sci USA. Nov. 22; 113(47):E7500-E7509. PMCID: PMC5127353 [ link ]
Srivastava V, Iglesias PA, Robinson DN*. 2016. Cytokinesis: Robust cell shape regulation. Semin. Cell Dev. Biol. 2016; 53:39-44.
Schiffhauer ES, Luo T, Mohan K, Srivastava V, Qian X, Griffis E, Iglesias PA, Robinson DN*. Mechanoaccumulative elements of the mammalian actin cytoskeleton. Curr. Biol. 2016; 26: 1473-1479.
Swaney K.F., Borleis J., Iglesias P.A., and Devreotes, P.N. 2015.  Novel protein Callipygian defines the back of migrating cells. PNAS 2015 Jun 30. pii:201509098. [Epub ahead of print]
Wang M, Artemenko Y, Cai W, Iglesias PA and Peter N. Devreotes. (2014). The Directional Response of Chemotactic Cells Depends on a Balance between Cytoskeletal Architecture and the External Gradient.  Cell Rep Nov 6;9(3):1110-21. doi: 10.1016/j.celrep.2014.09.047. Epub 2014 Oct 23. PMID: 25437564 [PubMed - in process]. PMCID:PMC4250826  
Tang M, Wang M, Shi C, Iglesias PA, Devreotes PN, and Huang CH  (2014).  Evolutionarily conserved coupling of adaptive and excitable networks mediates eukaryotic chemotaxis. Nat Commun. Oct 27;5:5175. PMCID: PMC4211273 [Available on 2015/4/27)]
Cai H, Katoh-Kurasawa M, Muramoto T, Santhanam B, Long Y, Li L, Ueda M, Iglesias PA, Shaulsky G, and Devreotes PN. Nucleocytoplasmic shuttling of a GATA transcription factor functions as a development timer. Science. 2014 Mar 21;343(6177):1249531. doi: 10.1126/science.1249531.
Huang, C.,Tang, M., Shi, C., Iglesias, P., and Devreotes, P.N. An excitable signal integrator couples to an idling cytoskeletal oscillator to drive cell migration. Nat Cell Biol. 2013 Nov;15(11):1307-16.
Luo T, Mohan K, Iglesias PA, Robinson DN. Molecular mechanisms of cellular mechanosensing. Nat. Mater. 2013; 12: 1064-1071.
Kee YS, Ren Y, Dorfman D, Iijima M, Firtel RA, Iglesias PA, Robinson DN. A mechanosensory system governs myosin II accumulation in dividing cells. Mol. Biol. Cell 2012; 23(8): 1510-1523.
Poirier CC, Ng WP, Robinson DN, Iglesias PA. Deconvolution of the cellular force-generating subsystems that govern cytokinesis furrow ingression. PLoS Comp. Biol. 2012; 8(4): e1002467. 
Luo T, Mohan K, Srivastava V, Ren Y, Iglesias PA, Robinson DN. Understanding the cooperative interactions between myosin II and actin crosslinkers mediated by actin filaments during mechanosensation. Biophys. J. 2012; 102(2): 238-247. 
Porter JR, Lee CSY, Espenshade PJ, Iglesias PA. 2012. Regulation of SREBP during hypoxia requires Ofd1-mediated control of both DNA binding and degradation. Mol. Biol. Cell 23:3764-3774.
Zhou Q, Kee Y-S, Poirier CC, Jelinek C, Osborne J, Divi S, Surcel A, Tran ME, Eggert US, Müller-Taubenberger A, Iglesias PA, Cotter RJ, Robinson DN. 14-3-3 coordinates microtubules, Rac, and myosin II to control cell mechanics and cytokinesis. Curr. Biol. 2010; 20:1881-1889.
Porter JR, Burg JS, Espenshade PJ, Iglesias PA. 2010. Ergosterol regulates SREBP cleavage in fission yeast. J. Biol. Chem. 285:41051-61.
Xiong, Y., Huang, C-H, Iglesias, P.A., and Devreotes, P.N. 2010. Cells navigate with a local-excitation, global-inhibition-biased excitable network. PNAS, 107:17079-17086.
Ren Y, Effler JC, Norstrom M, Luo T, Firtel RA, Iglesias PA, Rock, RS, Robinson DN. Mechanosensing through cooperative interactions between myosin-II and the actin crosslinker cortexillin-I. Curr. Biol. 2009; 19(17):1421-1428.
Reichl EM, Ren Y, Morphew MK, Delannoy M, Effler JC, Girard KD, Divi S, Iglesias PA, Kuo SC, Robinson DN.* 2008. Interactions between myosin and actin crosslinkers control cytokinesis contractility dynamics and mechanics. Curr. Biol. 18: 471-480. PMCID: PMC2361134
Chen, L., Iijima, M., Tang, M., Landree, M.A. Huang, Y.E., Xiong, Y., Iglesias, P.A., Devreotes, P.N. PLA2 and PI3K/PTEN pathways act in parallel to mediate chemotaxis. Developmental Cell. Developmental Cell. (2007) 12: 603-614.
Effler JC, Kee Y-S, Berk JM, Tran MN, Iglesias PA, Robinson DN.* Mitosis-specific mechanosensing and contractile protein redistribution control cell shape. Curr. Biol. 2006; 16(19):1962-1967. PMCID: PMC2474462
Janetopoulos C, Ma L, Devreotes PN, Iglesias PA. Chemoattractant-induced phosphatidylinositol 3,4,5-trisphosphate accumulation is spatially amplified and adapts, independent of the actin cytoskeleton. Proc Natl Acad Sci U S A. 2004 Jun 15;101(24):8951-6. PMC428453

Scot Kuo, Ph.D.

Scot Kuo, Ph.D.

Research Topic: Cell Motility and Cell Mechanics using novel laser-based optical tools

Our long-term goals are to understand cell motility, which is critical for immunological defense and wound healing, but unwanted during metastatic cancers. Because these processes are ultimately mechanical in nature, our focus is developing and applying new laser-based optical instrumentation with high-resolution microscopy imaging to understand cellular mechanics.

Selected Publications:
Yamanaka, S., Campbell, N.R., An, F., Kuo, S.C., Potter, J.J., Mezey, E., Maitra, A., and Selaru, F. (2012) Coordinated effects of microRNA-494 induce G2/M arrest in human cholangiocarcinoma. Cell Cycle 11, 2729-38
Fisher, C.I., and Kuo, S.C. (2009) Filament rigidity causes F-actin depletion from nonbinding surfaces. PNAS 106, 133-138
Girard, K.D., Kuo, S.C., and Robinson, D.N. (2006) Dictyostelium myosin-II mechanochemistry promotes active behavior of the cortex on long time-scales. PNAS 103, 2103-2108
Girard, K.D., Chaney, C., Delannoy, M., Kuo, S.C., and Robinson, D.N. (2004) Dynacortin contributes to cortical viscoelasticity and helps define the shape changes of cytokinesis. EMBO J 23(7), 1536-46
McGrath, J.L., Eungdamrong, J., Fisher, C.I., Peng, F., Mahadevan, L., Mitchison, T.J., and Kuo, S.C. (2003) The force-velocity relationship for the actin-based motility of Listeria monocytogenes. Curr Biol 13, 329-332
Kuo, S.C., and McGrath, J. L. (2000) Steps and fluctuations of Listeria monocytogenes during actin-based motility. Nature 407, 1026-9
Yamada, S., Wirtz, D., and Kuo, S.C. (2000) Mechanics of living cells measured by laser tracking microrheology (LTM). Biophys. J. 78, 1736-47.
Lab Members:
Namesort descending Classification Email Phone
Barbara Smith EM Specialist bsmit112@jhmi.edu 410-614-2086 (Office), 410-614-2135 (Lab)
Hoku West-Foyle, Ph.D. EM Specialist hwestfo1@jhu.edu 410-614-6890
LaToya Roker, Ph.D. EM Specialist lroker1@jhmi.edu 410-614-2135
Loza Lee Administrative Coordinator loza@jhmi.edu 410-614-6890
Mike Delannoy Sr. Laboratory Manager delannoy@jhmi.edu 410-955-1365 (Office), 410-614-2135 (Lab)